Table 1.
The sense and antisense primer sequences of the gene of interest for PCR amplification.
| Target gene | Primer sequence (5'? 3') | Amplicon size (bp) | Anneal. temp/ cycles |
|---|---|---|---|
| Bax | Sense: GACACCTGAGCTGACCTTGG Antisense: GAGGAAGTCCAGTGTCCAGC |
310 | 58 °C/35 |
| Bcl2 | Sense: GGGATGCCTTTGTGGAACTA Antisense: CTCACTTGTGGCCCAGGTAT |
138 | 56 °C/35 |
| Caspase 3 | Sense: AGTTGGACCCACCTTGTGAG Antisense: AGTCTGCAGCTCCTCCACAT |
298 | 55 °C/35 |
| ß-actin | Sense: TCATGCCATCCTGCGTCTGGACCT Antisense:CGGACTCATCGTACTCCTGCTTG |
598 | 55 °C/ 35 |