Figure 1.

Two candidate molecules for inhibiting Vif-mediated degradation of APOBEC3G were identified by a multi-round screening. A. Schematic depicting of the primary screening for Vif inhibitors using EGFP-fused APOBEC3G. B. Spreading infection of HIV-1 in the presence of the candidate small molecules. C. Structures of two candidate molecules share similar backbone. D. Fluorescence measurement (histogram) and immunoblot analyses (panels) of the effect of two candidate molecules on EGFP-fused APOBEC3G protein levels in the presence of Vif protein. 293 T cells transiently expressing EGFP-APOBEC3G in the presence of transient expression of Vif were treated with 10 μM MM-1 or MM-2 for 24 hours. Fluorescence intensity was measured and that of DMSO-treated cells without Vif expression was normalized to 100%. Average and standard error of three independent experiments are shown in the histogram.