Fig. 2.
Analysis of cell properties of cultured endometrial stromal cells. Total RNA was obtained from the cultured endometrial stromal cells, reverse-transcribed, and amplified by PCR using primers for cytokeratin 19, vimentin, ERα and ERβ (A, B). Each PCR product was electrophoresed and stained with ethidium bromide. Whole-cell extracts were subjected to Western blotting with rabbit polyclonal anti-ERα and anti-ERβ antibodies (C). The cells were treated with E2 (10−9 M) for 24 h. Igf1 mRNA levels were determined by real-time RT-PCR (D). Values are means ± SEM of triplicate wells. The expression of each mRNA was normalized for RpL19 mRNA expression. *P < 0.05; **P < 0.01 compared with the control.