Analysis of cell properties of cultured endometrial stromal cells. Total RNA was obtained from the cultured
endometrial stromal cells, reverse-transcribed, and amplified by PCR using primers for cytokeratin 19, vimentin, ERα
and ERβ (A, B). Each PCR product was electrophoresed and stained with ethidium bromide. Whole-cell extracts were
subjected to Western blotting with rabbit polyclonal anti-ERα and anti-ERβ antibodies (C). The cells were treated
with E2 (10−9 M) for 24 h. Igf1 mRNA levels were determined by real-time RT-PCR (D).
Values are means ± SEM of triplicate wells. The expression of each mRNA was normalized for RpL19
mRNA expression. *P < 0.05; **P < 0.01 compared with the control.