Skip to main content
. 2014 Mar 25;60(3):216–223. doi: 10.1262/jrd.2013-085

Fig. 3.

Fig. 3.

Effects of PPT, MPP, DPN and PHTPP on class 1 Igf1 mRNA expression in cultured endometrial stromal cells. (A) Endometrial stromal cells were treated with PPT (0, 10–9, 10–8, 10–7 M) for 24 h. (B) Endometrial stromal cells were treated with MPP (0, 10–9, 10–8, 10–7, 10–6 M) in the absence or presence of PPT (10–7 M) for 24 h. (C) Endometrial stromal cells were treated with DPN (0, 10–9, 10–8, 10–7 M) for 24 h. (D) Endometrial stromal cells were treated with PHTPP (0, 10–7, 10–6 M) or MPP (0, 10–7, 10–6 M) in the absence or presence of PPT (10–8 M) for 24 h. (E) Endometrial stromal cells were treated with MPP (10–6 M) or PHTPP (10–6 M) in the absence or presence of E2 (10–9 M) for 24 h. Igf1 mRNA levels were determined by real-time RT-PCR. Values are means ± SEM of triplicate wells. The expression of each mRNA was normalized for RpL19 mRNA expression. Independent experiments were performed three times, and similar results were obtained. *P < 0.05; **P < 0.01 and ***P < 0.001 compared with the control; #P < 0.05 compared with E2 (10–9 M) alone; ##P < 0.001 compared with PPT (10–7 M) alone or DPN (10–8 M) alone.