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. 2014 Mar 25;60(3):216–223. doi: 10.1262/jrd.2013-085

Table 1. Primer sequence used for RT-PCR and Real-time PCR.

Gene Sequence (5′–3′) Length (bp)
RT-PCR
Krt19
Forward GTGTCTGATGGGCTGCTGTCT 539
Reverse CTCAGGATCTTGGCTAGGTCG
Vim
Forward GGCCGAGGAATGGTACAAGTC 320
Reverse GGGCCATCTTAACATTGAGCAG
ERa
Forward CTAATTCTGACAATCGACGC 347
Reverse GTGCTTCAACATTCTCCCTCCTC
ERb
Forward GGTGTCTGGTCCTGTGAAGGATGT 670
Reverse CCGTCGTCGCCAGGAGCATGTCAA
RpL19
Forward GAAATCGCCAATGCCAACTC 406
Reverse TGAGGCTCGCAGGTCTAAGA

Real-time PCR
class1 Igf1
Forward GCAGCCTTCCAACTCAATTATTTAA 89
Reverse GTAGAAGAGGTGTGAAGACGACATG
class2 Igf1
Forward CACCTGTTCTTAAGTCCTCAGTTTTG 143
Reverse GTAGAAGAGGTGTGAAGACGACATG
RpL19
Forward CCGCAGCCATGAGTATGCT 60
Reverse CGCAGCGGAGGACACTAGA