Table 1. Primer sequence used for RT-PCR and Real-time PCR.
Gene | Sequence (5′–3′) | Length (bp) |
RT-PCR | ||
Krt19 | ||
Forward | GTGTCTGATGGGCTGCTGTCT | 539 |
Reverse | CTCAGGATCTTGGCTAGGTCG | |
Vim | ||
Forward | GGCCGAGGAATGGTACAAGTC | 320 |
Reverse | GGGCCATCTTAACATTGAGCAG | |
ERa | ||
Forward | CTAATTCTGACAATCGACGC | 347 |
Reverse | GTGCTTCAACATTCTCCCTCCTC | |
ERb | ||
Forward | GGTGTCTGGTCCTGTGAAGGATGT | 670 |
Reverse | CCGTCGTCGCCAGGAGCATGTCAA | |
RpL19 | ||
Forward | GAAATCGCCAATGCCAACTC | 406 |
Reverse | TGAGGCTCGCAGGTCTAAGA | |
Real-time PCR | ||
class1 Igf1 | ||
Forward | GCAGCCTTCCAACTCAATTATTTAA | 89 |
Reverse | GTAGAAGAGGTGTGAAGACGACATG | |
class2 Igf1 | ||
Forward | CACCTGTTCTTAAGTCCTCAGTTTTG | 143 |
Reverse | GTAGAAGAGGTGTGAAGACGACATG | |
RpL19 | ||
Forward | CCGCAGCCATGAGTATGCT | 60 |
Reverse | CGCAGCGGAGGACACTAGA |