Fig. 4.

CTSD-IFITM10 read-through fusion transcript siRNA knockdown. a We designed qPCR primers to flank the fusion junction of the CTSD-IFITM10 read-through fusion transcript and we designed two custom siRNAs to target the fusion junction. The sequence from the CTSD (the 5′ gene) is indicated in green and the sequence from IFITM10 (3′ gene) is indicated in red. The MCF7 breast cancer cell line was transfected with two siRNAs targeting the CTSD-IFITM10 fusion junction. b qPCR of the fusion transcript was performed 48 h after transfection. Both siRNAs significantly reduced the abundance of the fusion transcript relative to the controls, which included a non-targeting siRNA and a mock transfection that did not contain any siRNA. c A quantitative cell proliferation assay was performed 72 h after transfection. Both siRNAs significantly reduced the number of live cells relative to the controls