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. 2014 Mar 17;11(3):253–262. doi: 10.1038/cmi.2013.67

Figure 1.

Figure 1

Th17 cells are increased in human pregnancy. The percentages of IL-17-expressing CD4+ T cells were analyzed by intracellular cytokine assays. The results were gated on CD4 and MACS-sorted peripheral blood and decidual CD4+ T cells were stimulated with PMA, ionomycin and BFA for 4 hours. The surface marker of Th17 cells were analyzed by FACS. (a) The proportion of Th17 cells in PBMC during human pregnancy is compared with that in non-pregnancy (n=10, left); the phenotype of first-trimester human peripheral blood Th17 cells (right). (b) The proportion of Th17 cells in human first-trimester decidua is compared with non-pregnant endometrium of secretory phase (n=10, left); the phenotype of first-trimester human decidual Th17 cells (right). (c) Comparison of CD161 on Th17 cells between PBMC and deciduae. (d) Comparison of Th17 cells between PBMC and decidua in first-trimester human pregnancy. Error bars indicate the s.e.m. The FACS picture represents an individual sample. BFA, brefeldin A; FACS, Fluorescence-activated cell sorting; MACS, magnetic affinity cell sorting; PBMC, peripheral blood mononuclear cell; PMA, phorbol myristate acetate; s.e.m., standard error of the mean; Th17, T helper 17.