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VEGF induces VEGFR2 phosphorylation and activation of the downstream signal cascade. Pre-incubation of MIL60 and 50 ng/ml VEGF blocked its efficacy for activating key signal molecules such as VEGFR2, ERK, P38 and p65 in a dose-dependent manner. The MIL60 or Avastin antibodies were diluted to 0.04 µg/ml, 0.4 µg/ml and 4 µg/ml. VEGF, vascular endothelial growth factor.
