Table 1. Affinity determination of MIL60 using Biacore analysis.
Kon(1/Ms) | Koff (1/s) | KD (pM) | |
---|---|---|---|
Avastin | 3.15×105 | 1.40×10−5 | 44.5 |
MIL60 | 3.21×105 | 1.37×10−5 | 42.7 |
The KD value of MIL60 was 44.5 pM, which was nearly the same as that of Avastin (42.7 pM). KD=Kon/Koff.