Fig. 4.

Low intra-assay variability by ddPCR over a wide range of HTLV-1 PBMC PVL. The intra-assay variability of ddPCR for low, medium, and high PVL groups was evaluated by running ten replicates of PBMC DNA from eight patients (three AC and five HAM/TSP). For the low PVL group (<5 %), the mean PVL was 1.85 % with a mean CV of 13.7 %. For the medium PVL group (5–10 %), the mean PVL was 9.77 % with a mean CV of 7.44 %. For the high PVL group (>10 %), the mean PVL was 32.245 with a mean CV of 3.4 %