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. 2014 Mar 24;11(4):387–397. doi: 10.1038/cmi.2014.13

Figure 1.

Figure 1

IL-15-deficient NOD.scid mice accumulate more CD4+ T cells following adoptive transfer of NOD splenocytes. (a) Total splenocytes from diabetic NOD mice were adoptively transferred to NOD.scid, NOD.scid.Il15−/− and NOD.scid.gamma mice. Two months later, splenocytes from the recipient mice were counted and the frequency of CD4+ T cells was determined by FACS to determine the absolute number of CD4+ T cells recovered. (b) The numbers of CD4 T cells recovered from NOD.scid.Il15−/− and NOD.scid.gamma recipients two months after adoptive transfer (top panel) are expressed as fold-increase (n=6; mean±standard error of mean) over those recovered from NOD.scid recipients to offset variability in cell numbers between experiments (bottom panel). (c) Purified NOD CD4+ or CD8+ T cells were stimulated with IL-15 (10 ng/ml) for 15 min. Total cell lysates were analyzed by western blot for pSTAT5, stripped and blotted for total STAT5 protein. NOD, non-obese diabetic.