Figure 2.

Trans-presented IL-15 attenuates homeostatic expansion of CD4⁺ T cells in NOD.scid mice. (a) Total splenocytes from four weeks-old NOD mice were injected intravenously (1×10⁷ cells) into NOD.scid, NOD.scid.Il15^−/− or NOD.scid.gamma recipients. Three days after adoptive transfer, cell suspensions from spleen and MLNs were analyzed by flow cytometry to determine the CFSE-dye dilution in CD4⁺ or CD8⁺ T-cell compartments. Numbers in the graph indicate the proportion of cells within the marker boundaries. (b) CFSE-labeled NOD splenocytes were transferred to NOD.scid.Il15ra^−/− mice and recipient mice were analyzed 3 days later. Representative data from one animal for each recipient group (5–6 mice/group) from at least two independent experiments are shown. CFSE, 5-(6)carboxyfluorescein diacetate succinimidyl ester; MLN, mesenteric lymph node; NOD, non-obese diabetic.