Table 1. PCR and QPCR primers for murine genes.
Primer | Forward 5′→3′ | Reverse 5′→3′ |
---|---|---|
β-actin | GGTCTCAAACATGATCTGGG | GGGTCAGAAGGATTCCTATG |
IL-1Ra | GACCCTGCAAGATGCAAGCC | GAGCGGATGAAGGTAAAGCG |
Insulin | CAGAAACCATCAGCAAGCAGG | TTGACAAAAGCCTGGGTGGG |
IL-1β | CTCCATGAGCTTTGTACAAGG | TGCTGATGTACCAGTTGGGG |
TNF-α | CCTGAGTTCTGCAAAGGGAG | AGCAAAAGAGGAGGCAACAA |