Table 3. GMSP assay-generated profiles of skewed (oligoclonal or monoclonal) TRBV families in CD4+ and CD8+ T cells in subjects who have recovered from AHI.
| Subjects no. | CD4+ cells | CD8+ cells | ||
|---|---|---|---|---|
| TRBV oligoclonality | TRBV monoclonality | TRBV oligoclonality | TRBV monoclonality | |
| S1 | 8/9/22 | 14/17 | 5.1/7/14/15/20 | 4/8/11/12/13.1 |
| S2 | 3/7/15/18/20/24 | 11/12 | None | 1/15/20/21/22/24 |
| S3 | 15/16 | 10/13.1/23 | 11/12/15/17/19 | 2/5.1/6/7 |
| S4 | 9/22 | 7/19 | 13.2/22/24 | 3/4/10/15/18/20 |
| S5 | 3/5.1/11/21/23 | 2/7/15 | 5.1/9/13.1/14/17/21 | 11/19 |
| S6 | 5.2/9/24 | 4/8/11/13.1 | 9 | 4/13.1/17/20/22 |
| S7 | None | None | 6/18 | 5.1/5.2/7/8/19 |
| S8 | 3/10/11/12/19/21 | 15/24 | 19/23 | 11/14/20/24 |
| S9 | 12/20/22 | 18 | 3/13.2/15/20/22/24 | 9 |
| S10 | 1/4/11/14/24 | 8/20 | 1/5.2 | 13.2/18 |
| S11 | 6/14/24 | 4/5.1/12/20 | 5.1/19/23 | 10/12/13.1 |
| S12 | 8/13.2 | 1/9/24 | 4/8/10/18 | 2/11/15/23/24 |
| S13 | 2/5.1/13.1/17/21 | 13.2/19 | 2/20/24 | 7/11/17/19 |
| S14 | 7/10/13.1 | 5.2/23 | 4/8/11/12/13.1/15 | 5.1/16 |
| S15 | 5.2/6/14/15/16 | 7 | 3/21/23/24 | 13.2/20 |
| S16 | 10/17/23 | 18 | 7/11/13.1/16/24 | 5.1/20 |
| S17 | 3/5.1/11/13.1/20/22 | 4/12/14 | 1/5.1/10/14/17/20/22 | 12/23 |
| S18 | 15/18 | 6/8/11 | 3/5.2/19/24 | 8/11/13.1/15 |
| S19 | 2/10/16/19 | 12/13.2 | 2/11/13.2/20 | 24 |
| S20 | 15 | 5.1/13.1/20/23 | 5.1/12/15 | 1/11 |
| S21 | 2/5.2 | 8 | 1 | 6/16/18/22 |
| S22 | 11/18 | 5.2/24 | 7/10/15/20/23/24 | 2/5.1/13.2/21 |
| S23 | 7/12/21 | 1/10/13.1/15/18 | 3/6/11 | 9/15/17/18 |
| S24 | None | None | 7/16/18 | 1/5.2/11/13.1/14/23 |
| S25 | 4/5.2/8/13.2/20 | 14/15/19 | 2/5.1/11/15/21 | 6/24 |
| S26 | 6/15/22 | 1/5.1/18 | 10/14/21 | 2/5.1/7/20 |
| S27 | 2/12/23 | 9/15/21 | 4/13.1/16 | 12/24 |
| S28 | 5.1/11/17/18/20/21 | None | 8/12/18/23/24 | 6/11/13.1/16 |
| S29 | 15 | 12/13.2 | 17 | 4/8/15/22 |
| S30 | 21 | 5.1/20/22 | 5.2/9/11 | 3/14/20/24 |
| Total no. of skewed TRBV families (no. of subjects) | 95 (30)a | 68 (30)b | 108 (30)a | 105 (30)b |
Abbreviations: AHI, acute hepatitis B virus infection; GMSP, gene melting spectral pattern; PBMC, peripheral blood mononuclear cell; TRBV, T-cell receptor beta-chain variable.
a
The ratio of oligoclonal-expanded TRBV families was not significantly different between the two groups (P>0.05 by χ2 test).
b
The monoclonal ratio was lower in the CD4+ cells than that in the CD8+ cells (P<0.01 by χ2 test).
None, no skewed TRBV family was detected.