Table 5. Representative amino acid sequences of monoclonal TRBV gene families in PBMCs from subjects who have recovered from AHI.
| Subjects | Vbeta | CDR3 | BJ | Ratio | ||
|---|---|---|---|---|---|---|
| S1 | BV20 | SGFYLCAWS | TGTGHTPV | HFGNGTRLTVTED | 1.6 | 15/20 |
| S15 | BV20 | SGFYLCAWS | TGTGHSPL | HFGNGTRLTVTED | 1.6 | 18/21 |
| S27 | BV20 | SGFYLCAWS | VQNEQ | FFGPGTRLTVLED | 2.1 | 17/20 |
| S6 | BV18 | SAAYFCASS | RTGDTEA | FFGQGTRLTVVED | 1.1 | 23/23 |
| S3 | BV17 | TAFYLCASS | RQLTEA | FFGQGTRLTVVED | 1.1 | 19/23 |
| S1 | BV15 | TALYFCATS | RFAYGRTNEQ | FFGPGTRLTALED | 2.1 | 24/27 |
| S9 | BV13.2 | TSVYFCASS | YSTDEQ | YFGPGTRLTVTED | 2.7 | 21/24 |
| S12 | BV13.1 | TSVYFCAST | PGYSYEQ | YFGPGTRLTVTED | 2.7 | 20/23 |
| S5 | BV12 | TSVYFCAIR | KQGDSYEQ | YFGPGTRLTVTED | 2.7 | 19/23 |
| S9 | BV12 | TSVYFCASC | PGGSGGTGEL | FFGEGSRLTVLED | 2.2 | 20/23 |
| S2 | BV11 | TSQYLCASS | PGEL | FFGEGSRLTVLED | 2.2 | 21/23 |
| S15 | BV11 | TSQYLCASS | AGEL | FFGEGSRLTVLED | 2.2 | 20/23 |
| S21 | BV11 | TSQYLCATG | VYNEQ | FFGPGTRLTVLED | 2.1 | 20/23 |
| S16 | BV9 | SAVYFCASS | PWNTGEL | FFGEGSRLTVLED | 2.2 | 18/23 |
| S32 | BV5.1 | SALYLCASS | LDYSLYTI | YFGEGSWLTVVED | 1.3 | 20/23 |
| S15 | BV3 | TSMYLCASS | LGRETQ | YFGPGTRLLVPED | 2.5 | 17/21 |
| S32 | BV3 | TSMYLCASS | FLLRGMFFYEQ | YFGPGTRLTVTED | 2.7 | 18/23 |
Abbreviations: AHI, acute hepatitis B virus infection; BJ, T-cell receptor beta joint region; CDR3, complementary determining regions; PBMC, peripheral blood mononuclear cell; TRBV, T-cell receptor beta-chain variable; Vbeta, T-cell receptor beta-chain variable region.
When the GMSP of a TRBV gene family displayed a single peak (Figure 5), the PCR product of the corresponding TRBV gene family was re-amplified, and the PCR product was sequenced after cloning. Only the amino-acid sequences of the CDR3 max ratios are shown. The identical sequences are underlined.