Figure 1.

Identification of tumorigenic cells in KrasG12D-induced lung carcinoma. (a) FACS analysis of the purified LSL KrasG12D p53^KO and KrasG12D p53^KO lung epithelial cells using the indicated antibodies. (b, c) Western blot analysis of KrasG12D expression, (b) or pull-down of GTP-bound active Kras (c) in LSL KrasG12D p53^KO cells (inactive KrasG12D allele), KrasG12D p53^KO cells (active KrasG12D allele) or tumors derived from these cells. Passage numbers are indicated. MAPK is a loading control. (d) Genomic PCR of LSL KrasG12D p53^KO cells infected with Cre-expressing retroviruses distinguishes recombined KrasG12D allele from WT Kras allele by addition of 40 bp in the intronic region. The nonrecombined LSL KrasG12D allele does not amplify under these conditions. (e) Representative images of tumors from mice injected with CD34− and CD34+ KrasG12D p53^KO cells