Figure 5.

ARHI induces ATG4 and MAPLC3 expression and is mediated through FOXo3a. (a) SKOv3-ARHI cells were treated with or without DOX for the indicated intervals before cells were harvested. Cell lysates were probed with antibodies against LC3, ATG4, ARHI and actin. Band intensities were quantified and expressed relative to the untreated controls in which a value of 1.0 was assigned. (b) Cells were treated as in a and the levels of LC3B and ATG4 mRNAs were quantified by real-time RT-PCR. The columns indicate the mean, and the bars indicate the S.E. (*P<0.05; **P<0.01). Data were obtained from three independent experiments. (c) SKOv3-ARHI cells were transfected with siControl (C) or siFOXo3a (F) and treated with or without DOX. The columns indicate the mean, and the bars indicate the S.E. (**P<0.01). Data were obtained from three independent experiments. (d) Efficient knockdown of FOXo3a