Figure 6.

Cellular Keap1 protein levels regulate PA-induced JNK activation and Bim and PUMA upregulation in liver cells. (a–e), Whole-cell lysates were prepared from shLuc or shKeap1#4 Hep3B cells treated with PA at 400 and 800 μM or vehicle (V) for 6 h (a), from shLuc or shKeap1#4 Hep3B cells treated with PA at 600 μM at the indicated time point (b), from WT or hepatocyte specific Keap1 knockout (Keap1^−/− HKO) primary mouse hepatocytes treated with PA at 600 μM for the indicated time points (c–d) or from Hep3B cells stably transfected with Keap1 C-terminal deletion mutant (Keap1 ΔCTR) or with the control lentiviral plasmid (control) and treated with PA 400 μM at the indicated time points (e). Immunoblot analysis were performed for phosphorylated JNK (p-JNK), total JNK (t-JNK), Bim, PUMA, Bcl-_XL and Mcl-1. Tubulin or β-actin were used as a control for protein loading. Bands were cut and combined (separated by dotted line) from the same radiograph