Figure 8.

Fluorescently labeled VAV3 peptide is internalized into GICs. (a–d) GICs and non-GICs from tumor specimens 08-387 and 4121 were treated with the green fluorescent-tagged 5-Fap peptide that corresponds to the VAV3 protein. The nuclear counterstain DAPI is in blue and scale bar=10 μm. (e) Bulk, unsorted cells from three glioblastoma specimens (3691, 4121 and GBM10) were treated with green 5-Fap VAV3 peptide and then sorted based on peptide uptake by FACS into 96-well plates for a limited dilution sphere formation assay