Figure 2.

Immunolocalization of FcRn in polarized T84 monolayers (a–c) and normal adult human small intestinal mucosa (d–g). (a) Whole-mount T84 monolayers show a diffuse, punctate staining pattern. The Z0-1 image was captured slightly above the focal plane of FcRn. (b) FcRn staining of whole-mount T84 monolayers viewed as confocal vertical sections. (c) FcRn staining was absent in the presence of an isotype-matched, irrelevant antiserum. (f) Villous enterocytes of normal adult human small intestine show delicate linear staining in the region of the apical cytoplasmic membrane. (g) Crypt enterocytes show an apical and punctate staining pattern visible not only at the level of the apical cytoplasmic membrane, but also in the apical cytoplasm below the level of the apical membrane. (d and e) FcRn staining was absent in the presence of an irrelevant antiserum or with secondary antibody alone (not shown).