Figure 1.

HRV-14 inhibits antigen-specific T-cell proliferation. PBMCs (10⁵) were stimulated with mAb OKT3 (1 μg/mL), SEA or SEB (10 ng/mL), and PPD or tetanus toxoid (1 μg/mL) in the presence (filled bars) or absence (gray bars) of HRV-14 (10 TCID₅₀ per cell). T-cell proliferation was measured on day 3 (for OKT3, SEA, SEB stimulation) and day 5 (for PPD, tetanus toxoid stimulation) of culture by adding (methyl-³H)-TdR followed by measuring thymidine incorporation 18 hours later. Background counts due to ongoing autologous MLR in the absence of specific stimulation were subtracted. The figure shows thymidine uptake, in cpm (mean ± SEM) of 3 independent experiments. Paired Student’s t test was applied for statistical evaluation of data; corresponding P values are indicated.