Figure 2.

Inhibition of allogeneic T-cell proliferation by HRV-14. (a) Purified T cells (10⁵) were incubated with graded numbers of allogeneic, purified monocytes in the presence of HRV-14 (10 TCID₅₀ per cell; filled circles), control HeLa cell culture supernatants (open triangles), or medium alone (open circles). Proliferation of T cells was monitored on day 5 of culture by adding (methyl-³H)-TdR followed by measuring thymidine incorporation 18 hours later. The results shown are representative of 5 independent experiments. (b) Pretreatment of HRV-14 with WIN 52035-2 (5 μg/mL) for 30 minutes abolishes the inhibitory effect of HRV-14 (filled triangles). Addition of WIN 52035-2 (5 μg/mL) alone (open triangles) did not influence T-cell proliferation. The results are representative of 2 independent experiments. (c) Dose responses of HRV-14 particles on monocyte-induced allogeneic T-cell proliferation. The figure shows mean values ± SEM of 3 experiments.