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. 1999 Oct 1;104(7):957–965. doi: 10.1172/JCI7255

Figure 6.

Figure 6

Inhibition of IL-12 production by HRV-14. Purified monocytes (1 × 106/mL) were cultured for 2 days in the absence or presence of HRV-14 (10 TCID50 per cell) or LPS (100 ng/mL) with or without prestimulation with IFN-γ (300 U/mL) for 24 hours. Supernatants of cultured monocytes were analyzed for IL-12 p70 release by ELISA (a). The figure shows mean values ± SEM of 3 experiments. (b) Cytoplasmic staining of monocytes with PE-conjugated anti-IL-12 mAb or anti-IL-6 mAb. Monocytes were stimulated as follows: mock stimulated (A); +IFN-γ/LPS (B and G); +HRV-14/IFN-γ/LPS (C and H); +HRV-14/anti–IL-10 antibody/IFN-γ/LPS (D); +IL-10 (5 ng/mL)/IFN-γ/LPS (E); and +IL-10/anti–IL-10 antibody/IFN-γ/LPS (F).