Figure 4.

Ifnb mRNA Levels in Ifih1-Deficient MEFs Expressing IFIH1 Mutants

The MEFs were infected with retroviruses encoding mouse wild-type Ifih1, mouse Ifih1 with c.2461G>A (p.Gly821Ser) (RefSeq NM_027835.3) mutation, or the three AGS mutants of human IFIH1. At 48 hr after the retroviral infection, these MEFs were infected with indicated multiplicity of infection (MOI) of EMCV for 6 hr, and Ifnb mRNA levels were measured by qRT-PCR. The relative abundance of each transcript was normalized to the expression level of 18S ribosomal RNA. Data are shown as mean ± SEM of triplicate samples. Shown is a representative of two independent experiments. Statistical significance was determined by Student’s t test, ^∗p < 0.001. The expression of the retrovirally transduced FLAG-tagged constructs was confirmed by immunoblotting (Figure S5).