Fig. (3).

LSMMG-mediated alteration of acid stress resistance in Enterobacteriaceae. The indicated strains were grown in the RWV in the LSMMG and control conditions for testing of acid stress resistance (pH=3.5, citric acid, 2 hours stress exposure) as previously described [3, 7]. To test survival of the stress in the LSMMG and control samples, aliquots from each sample were removed from the stress, serially-diluted in PBS, and plated for CFU counts on LB media. The percent survival of the bacteria compared to the number present at t=0 before addition of the stress was determined for each sample, and then a ratio of percent survival values was calculated and plotted as indicated in the graph (either control/control or LSMMG/control). The data was obtained from at least three independent experiments each plated in triplicate, and the average and standard deviation are plotted. The differences between LSMMG and control were significant at p-value < 0.05.