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. 2014 Apr 16;3(2):320–332. doi: 10.3390/biology3020320

Figure 2.

Figure 2

Workflow of the immunoprecipitation (IP) in-solution digestion protocol described. Cells or tissues are lysed; the bait protein is enriched by IP (tagged or endogenous). IPs were washed, eluted, and in-solution digested. Peptides are separated and analysed on a reverse phase LC coupled to a high resolution mass spectrometer.