Table 2. Morphometric analysis of the Golgi apparatus by electron microscopy.
| Cells | GlcNAcT-V protein | Golgi cisternal stacka | Number of stacks/cell cross section | Size of cell profle in cross sectionsa (μm2) | Volume density of GAa (×10−3) | ||
|---|---|---|---|---|---|---|---|
|
| |||||||
| Number of cisternae | Length (μm) | Thickness (μm) | |||||
| Pro−5 | |||||||
| Exp. 1 (n = 35) | + | 5.1 ± 0.1 | 0.71 ± 0.03 | 0.19 ± 0.01 | 1.7 | 54.7 ± 4.0b | 5.1 ± 0.6c |
| Exp. 2 (n = 67) | + | 4.7 ± 0.1 | 0.68 ± 0.03 | 0.21 ± 0.01 | 1.6 | 61.9 ± 4.5b | 4.1 ± 0.5c |
| Lec4 | |||||||
| Exp. 1 (n = 30) | - | 5.0 ± 0.1 | 0.64 ± 0.04 | 0.18 ± 0.01 | 1.1 | 54.9 ± 3.4 | 3.2 ± 0.7 |
| Exp. 2 (n = 50) | - | 4.5 ± 0.1 | 0.61 ± 0.03 | 0.20 ± 0.01 | 1.1 | 65.4 ± 2.9 | 2.4 ± 0.3 |
| Lec4 GnTV-N5 | |||||||
| Exp. 1 (n = 36) | + | 5.0 ± 0.1 | 0.61 ± 0.03 | 0.17 ± 0.01 | 1.9 | 43.1 ± 2.4b | 5.5 ± 0.7c |
| Exp. 2 (n = 71) | + | 4.7 ± 0.1 | 0.57 ± 0.02 | 0.22 ± 0.01 | 1.7 | 49.3 ± 3.4b | 4.7 ± 0.5c |
a
Data are expressed as mean ± SEM
b
Indicates significant difference as compared with Lec4 cells (p < 0.01, Wilcoxon test) within the same experiment
c
Indicates significant difference as compared with Lec4 cells (p < 0.05, Wilcoxon test) within the same experiment