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Adenovirus-mediated production of biologically active CT-1. Conditioned media from AdCT-1– or AdLacZ-infected human fibroblasts were tested for their trophic activity on cultured motoneurons. The number of surviving motoneurons was determined 48 hours after plating and is represented as a function of the number of infectious particles per fibroblast (PFU/cell) or as a function of recombinant CT-1 protein (rCT-1; inset). Each point represents the mean of duplicate plates.
