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. 2014 Jul 7;206(1):45–59. doi: 10.1083/jcb.201312107

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© 2014 Zaytsev et al.

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Figure 6. — Analysis of contribution of other kinetochore MAPs to KMT affinity. (A) Relative K-fiber size predicted for different molecular lawns. The green curve is the total kinetochore affinity, and it is a sum of the contributions from NDC80 complexes with different degrees of phosphorylation and MAPs. (B) Relative K-fiber size predicted for different phosphomutants in the repetitive sites model. Contribution from NDC80 complexes was renormalized (compare with Fig. 4 D), such that the predicted number of KMTs did not exceed the experimentally measured K-fiber size. The MAP contribution is a difference between the best fit with the lawn model (A) and the contribution from NDC80 complexes. The combined affinity in the repetitive sites model was thus matched to the combined affinity in the lawn model (A). (C) Percentage of contribution of NDC80 complexes to the total KMT affinity calculated for the two models with MAPs. Both models provide a match to K-fiber size in cells (see A and B), but they predict different relative NDC80 versus MAP contribution. (D) Relative K-fiber size for kinetochores with depleted Nuf2 (a subunit of the NDC80 complex) in vivo (data from DeLuca et al., 2005) and predictions of the two models in which only MAPs are present. Experimental and theoretical data were normalized to the number of KMTs for WT cells. (E) Summary diagram of the tunable KMT interface. NDC80 complexes and other MAPs comprise a molecular lawn that interacts dynamically with competing MTs. Early in mitosis, NDC80 is likely to be hyperphosphorylated, so other MAPs provide a major contribution to the overall weak KMT binding. As MTs begin to attach, the mean phosphorylation on the Hec1 tail decreases to a range of one to two phosphates, and the contribution of NDC80 becomes significant. Further dephosphorylation in metaphase reduces the mean number of phosphates to a range of zero to one, and the contribution of NDC80 complexes to MT binding dominates. p indicates the number of phosphates. This drawing underrepresents the number of MT-bound NDC80 complexes, which is estimated to be 10–12 (Fig. S5 B). Error bars are SEMs.