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CXCR4 mRNA expression in HT-29 cells is reduced by sodium butyrate. HT-29 cells were serum starved for 24 hours and then stimulated with 1 mM or 5 mM sodium butyrate as shown for 3–24 hours. RNA was extracted as described in Methods and analyzed by Northern blotting. Blots were probed for CXCR4 mRNA (top panel) and β-actin to demonstrate equal loading. The result shown is representative of 3 similar experiments.
