Figure 1.

a) Experimental design. Identification of immunogenic proteins was based on b) autoantibody reactivity to arrayed MCF7 fractions. Peaks were determined qualitatively from microarray data. c) Western blots of individual fractions within a cluster with individual plasma samples were used to determine the molecular weight of reactive bands whose pattern qualitatively matches the microarray response pattern. d) Mass spectrometry analysis of fractions within a cluster identifies proteins by total peptide counts that match microarray and western blot data.