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. 1999 Oct 15;104(8):1123–1130. doi: 10.1172/JCI7308

Figure 6.

Figure 6

CREB independence of PKA activation of the hGH promoter. (a) CREB-deficient F9 cells were transfected with SV-40 expression vectors (pSG5) containing Pit-1 or CBP cDNAs and 195 bp of the proximal hGH promoter in the presence of WT or mutant PKA catalytic subunit. Data are expressed as fold activation relative to EV Pit-1 plus EV CBP plus PKA mutant. (b) CREB-deficient F9 cells and CREB-sufficient CV-1 cells were transfected with a common glycoprotein α-subunit reporter construct, which contains 2 well-defined CREs, and WT or mutant PKA catalytic subunit. Data are expressed as mean fold activation ± SEM relative to EV Pit-1 plus EV CBP plus PKA mutant.