a, Jhdm2a knockout mice exhibit an obesity phenotype. Shown is a representative photograph of 7-month-old littermates. b, Abnormal fat accumulation in organs of Jhdm2a−/− mice. Haematoxylin and eosin (H&E) staining of white adipose, muscle and liver derived from 7-month-old littermates. Arrowheads indicate the intramuscle fat deposition. Scale bars, 50 μm. c, Jhdm2a deficiency results in hyperlipidemia as indicated by the increased levels of serum free fatty acid (FFA), triglyceride (TG) and total cholesterol (TCHO) in Jhdm2a−/− (n = 16) compared with Jhdm2a+/+ (n = 10) mice. *P < 0.05; **P < 0.01. d, Growth curve of littermates fed with a high-fat diet (HFD) or normal chow. Solid lines, high-fat diet group; dotted lines, normal chow group. BW, body weight. n = 4 per group; *P < 0.03. Data are mean and s.e.m.