Figure 4.

Th1 and Th2 cells cooperate to induce high levels of endothelial VCAM-1 expression and tissue eosinophilia. Mice received transferred T cells and were challenged as described in Figure 1. Two days after the challenge, the mice were sacrificed and lung tissue was collected and frozen. Sections of tissue were then cut and stained for the presence of VCAM-1 (red) and eosinophils (brown) as described in Methods. Shown are representative sections of lung from mice that received (a) no transferred cells, (b) Th1 cells, (c) Th2 cells, or (d) Th1 and Th2 cells. Similar results were seen in 4 separate experiments.