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. 2014 Jan-Feb;56(1):29–33. doi: 10.1590/S0036-46652014000100004

Table 3. Mean results of each extraction method regarding parameters evaluated.

Parameter Boiling Phenol-chloroform Extraction column Salting out p
Agarose gel electrophoresis Variable* Variable* Positive Variable*
Amount of DNA in ng/µL 1018.2 (168.8) 87.8 (70.2) 65.5 (64.5) 28.2 (32.5) < 0.0001
A260/280 0.42 (0.03) 1.42 (0.15) 1.95 (0.26) 1.82 (0.44) < 0.0001
A260/230 2.99 (0.72) 0.66 (0.31) 1.34 (0.66) 0.81 (0.45) < 0.0001
PCR Positive Positive Positive Positive
Yeld 200 µL 100 µL 200 µL 100 µL
*

Some samples showed no visible bands; ( ) = Standard deviation.