Table 3. Mean results of each extraction method regarding parameters evaluated.
| Parameter | Boiling | Phenol-chloroform | Extraction column | Salting out | p |
|---|---|---|---|---|---|
| Agarose gel electrophoresis | Variable* | Variable* | Positive | Variable* | |
| Amount of DNA in ng/µL | 1018.2 (168.8) | 87.8 (70.2) | 65.5 (64.5) | 28.2 (32.5) | < 0.0001 |
| A260/280 | 0.42 (0.03) | 1.42 (0.15) | 1.95 (0.26) | 1.82 (0.44) | < 0.0001 |
| A260/230 | 2.99 (0.72) | 0.66 (0.31) | 1.34 (0.66) | 0.81 (0.45) | < 0.0001 |
| PCR | Positive | Positive | Positive | Positive | |
| Yeld | 200 µL | 100 µL | 200 µL | 100 µL |
*
Some samples showed no visible bands; ( ) = Standard deviation.