Fig. 5.

BvgA is not necessary for diguanylate-cyclase-dependent biofilm formation. (a). Quantitative measurements of cellular c-di-GMP from B. bronchiseptica grown planktonically in BGA supplemented with different NA concentrations. Formic-acid-extracted c-di-GMP was measured by LC-MS and normalized to mg dry weight of bacteria after extraction. (b) Biofilm formation by BbWT, Bb-DGC, BbΔBvgA and BbΔBvgA-DGC cultured in SS medium either alone or supplemented with NA was analysed by CV staining after 24 h in static conditions. (c) SEM of 48 h air–liquid interface submerged coverslips in SS medium alone (left panels), SS supplemented with 1.0 mM NA (middle panels) or SS supplemented with 4.0 mM NA (right panels). * P<0.01 vs BbΔBvgA grown at the same NA concentration.