Fig. 7.

Motility and biofilm phenotypes of B. bronchiseptica overexpressing BB3576. (a) mRNA transcripts levels of BB3576 were quantified by qPCR compared with the constitutive recA gene. Values were normalized to levels observed in SS media without additional NA. (b) The diameters of migration zones of the wild-type strain with pBBR1MCS-5 plasmid alone (Bb-pEmpty) or with the BB3576 gene (Bb-BB3576) were measured and expressed relative to Bb-pEmpty vector diameter after 18 h of incubation at 37 °C on SS motility plates supplemented with 32 mM magnesium sulfate. One hundred per cent motility for Bb-pEmpty was 12 mm. The results are based on three replicates. * P<0.01 vs Bb-pEmpty. (c) Biofilm development by Bb-BB3576 compared with Bb-pEmpty was assessed by microtitre plate assays as described in Methods. Biofilm level was determined in a ΔBvgA background (BbΔBvgA-BB3576 and BbΔBvgA-pEmpty). * P<0.01 vs Bb-pEmpty grown at the same modulator concentration, ** P<0.01 vs BbΔBvgA-pEmpty grown at the same modulator concentration.