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. 2014 Aug 1;21(4):533–550. doi: 10.1089/ars.2012.5129

FIG. 2.

FIG. 2.

Increased neuronal death in the hippocampus of 2VO-operated rats. (A) Representative fluorescence micrographs of DNA oxidation as visualized by 8-oxo-dG immunoreactivity (red) in the hippocampus (HP) at 10 weeks post-2VO operation. (B) Changes in the % by 8-oxo-dG (red)-stained neurons in the hippocampal CA1 subfield after sham- and 2VO operation in rats (n=4 per group, Error bars indicate SE). *p<0.05 versus Sham-operated rats. Representative images of tissue sections immunostained with NeuN (C), stained with Cresyl violet (E), and immunostained with cleaved caspase-3 (G) from rat hippocampal CA1 taken from sham-operated group (Sham control, n=4) and 2VO group (n=4) at 10 weeks after operation. (D) Changes in the % NeuN (red)-stained neurons of sham control and the number of NeuN (red)-stained neurons in the hippocampal CA1 subfield after sham- and 2VO operation in rats (n=4 per group, Error bars indicate SE). *p<0.05 versus sham-operated rats. (F) Changes in the % Cresyl violet-stained neurons of sham control and the number of Cresyl violet-stained neurons in the hippocampal CA1 subfield after sham- and 2VO operation in rats (n=4 per group, Error bars indicate SE). ***p<0.001 versus sham-operated rats. (H) Changes in the % cleaved caspase-3 (red)-stained neurons of NeuN (green)-stained cells and the number of cleaved caspase-3 and NeuN-stained neurons (yellow) in the hippocampal CA1 subfield after sham- and 2VO operation in rats (n=4 per group, Error bars indicate SE). **p<0.01 versus sham-operated rats. (I) Western blot analysis showing NeuN, MAP-2, and GFAP levels in the HP of the sham control (n=3) or 2VO group (n=3) at 10 weeks after operation. (J) The intensity of each band was densitometrically determined and normalized against β-actin. NeuN and MAP-2 levels were statistically decreased in 2VO-operated rats (n=3, **p<0.01 and ***p<0.001 vs. sham-operated rats). GFAP level significantly increased in 2VO-operated rats. 8-oxo-dG, 8-hydroxy-2′-deoxyguanosine; GFAP, glial fibrillary acidic protein. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars

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