Table 1.

Ts mutant position as predicted by sequence-based, structured-based or both methods

Protein	PDB ID	Chain length	Residue position	Residue type	Prediction method
gene V	1YHA	87	35	VAL	Both
			45	VAL	Both
			47	ILE	Structure
			63	VAL	Structure
			81	LEU	Structure
			78	ILE	Sequence
lambda repressor	1LMB	92	51	PHE	Both
			65	LEU	Both
			76	PHE	Both
			84	ILE	Both
			18	LEU	Structure
			36	VAL	Structure
			47	VAL	Structure
T4 lysozyme	2LZM	164	6	MET	Both
			102	MET	Both
			149	VAL	Structure
			153	PHE	Structure
			103	VAL	Sequence
CcdB	3VUB	101	17	PHE	Both
			18	VAL	Both
			33	VAL	Both
			34	ILE	Both
			54	VAL	Both
			5	VAL	Structure
			36	LEU	Structure
			63	MET	Structure
			50	LEU	Sequence
			53*	VAL	Sequence
			96	LEU	Sequence
			97	MET	Sequence
			98	PHE	Sequence
Gal4	3COQ	88	68	PHE	Both
			69	LEU	Sequence
			70	LEU	Sequence
Ura3	1DQW	267	25	MET	Structure
			32	LEU	Structure
			118	ILE	Structure

The wild-type residue (three-letter amino acid code) at the position is listed under residue type. * The prediction of VAL53 in CcdB as a Ts mutant position is a false positive identification.