Table 2.
(A) Comparison of previously reported in vivo fluxes and growth rates on aerobic and anaerobic conditions. Uptake and excretion fluxes are reported in mmol/g dry weight/h and growth rates in h - 1. Glucose was used as the limiting substrate. (B) Top 10 most significant reporter metabolites obtained from the reporter metabolites analysis for S. cerevisiae on minimal chemostat medium while changing from aerobic to anaerobic growth.
| A | ||||
|---|---|---|---|---|
| BioMet Toolbox v2 | ||||
| In vivo | In silico simulation | |||
| Aerobic | ||||
| Carbon limited aerobic glucose uptake | 1.15 | 1 | ||
| Carbon limited aerobic oxygen uptake | 2.7 | 2.74 | ||
| Carbon limited aerobic CO2 excretion | 2.8 | 2.78 | ||
| Carbon limited aerobic ethanol production | 0 | 0 | ||
| Carbon limited aerobic growth rate | 0.1 | 0.09 | ||
| Anaerobic | ||||
| Carbon limited anaerobic glucose uptake | 2.3 | 2 | ||
| Carbon limited anaerobic oxygen uptake | 0 | 0 | ||
| Carbon limited anaerobic CO2 excretion | 3.8 | 3.4 | ||
| Carbon limited anaerobic ethanol production | 3 | 3.4 | ||
| Carbon limited anaerobic growth rate | 0.03 | 0.04 | ||
| B | ||||
| Top 10 Reporter metabolites | ||||
| ferricytochrome c [mitochondrion] | ADP [mitochondrion] | |||
| ferrocytochrome c [mitochondrion] | H+ [mitochondrion] | |||
| ubiquinone-6 [mitochondrion] | urea [cytoplasm] | |||
| ubiquinol-6 [mitochondrion] | allantoate [cytoplasm] | |||
| phosphate [mitochondrion] | L-cysteine [extracellular] | |||