Figure 2. Properties of Ca²⁺ signals in s.l. astrocytes.

A. Image of a single s.l astrocyte expressing GCaMP3 with 11 ROIs indicated (Supp movie 1). B. Distributions of astrocyte Ca²⁺ signal properties (blue bars are for branches and the red bars are for somata). C. Traces for 11 ROIs from Fig 2A. D. Plots Ca²⁺ signal amplitude (left axis) and number (right axis) in 5 μm bins as a function of distance from the soma. The number of Ca²⁺ signals in 5 μm bins is shown by the black line. E. Image of an s.l. astrocyte with a yellow line indicating the approximate position of the region chosen for 200 Hz line scan imaging. F. Image of line scan data for the line shown in E, with an expanded region corresponding to a branchlet shown below. In these images, the x-axis is time and the y-axis is distance along the scanned line. G. The trace for the selected region shown in F. H. Distributions showing Ca²⁺ signal half widths from line scan experiments for somatic and branchlet regions. I-J. Bar graphs summarize Ca²⁺ signal properties such as dF/F and half width for line scan data. Average data are shown as mean ± S.EM.