Figure 5. Stratum lucidum region astrocyte Ca²⁺ responses during glutamate release from mossy fibers in SPRAE^hom mice.

A. IHC for P2X2-YC in the mossy fibers (green) along with staining for astrocytes using GFAP (red); higher magnification views shown in panel B. C. Three representative traces superimposed for ROIs from astrocyte branches from a WT mouse before, during and after 100 μM ATPγS applications. D. As in C, but for astrocytes located in the s.l. region and imaged from SPRAE^hom mice. E. Properties of astrocyte Ca²⁺ signals before and during ATPγS applications in WT mice. F. Properties of astrocyte Ca²⁺ signals before and during ATPγS applications in SPRAE mice. However, there was no significant difference in the duration of the spontaneous Ca²⁺ transients in WT and SPRAE mice before ATPγS. For somata, in WT mice their T_0.5 was 6.2 ± 0.9 s and in SPRAE mice the T_0.5 was 4.3 ± 1.1 s (n = 27 and 15; p = 0.16 with an unpaired t test). For branches, in WT mice their T_0.5 was 3.5 ± 0.2 s and in SPRAE mice the T_0.5 was 3.4 ± 0.4 s (n = 213 and 246; p = 0.83 with an unpaired t test). G. S.l. region astrocyte cell surface glutamate imaging with iGluSnFR in WT and SPRAE^hom mice. Average data are shown as mean ± S.EM.