. Author manuscript; available in PMC: 2015 Jul 1.

Published in final edited form as: Mol Microbiol. 2014 May 23;93(1):113–128. doi: 10.1111/mmi.12643

Table 2.

Muropeptide analysis of peptidoglycan from Pseudomonas aeruginosa PA14 WT and ΔrlpA grown in LB0N

Muropeptide	Structure^b	Observed m/z [M+Na⁺]	Expected m/z [M+Na⁺]	% of all peaks^c		% difference
Muropeptide	Structure^b	Observed m/z [M+Na⁺]	Expected m/z [M+Na⁺]	WT	ΔrlpA	% difference
4	DS-Tri	893.5	893.4	8.3 (0.2)	4.4 (1.8)	−47.0
5	TS	999.5	999.3	0.9 (0.6)	2.7 (0.7)	200.0
7	DS-Tetra	964.5	964.4	35.1 (1.2)	39.7 (2.4)	13.1
8	DS-Penta	1021.6	1021.4	3.4 (0.3)	2.0 (0.3)	−41.2
9	HS	1477.7	1478.4	0.9 (0.6)	2.5 (1.2)	177.8
11	DS-Tetra-Tetra	1407.7	1407.6	1.9 (0.6)	1.7 (0.6)	−10.5
13	TS-Tetra	1442.9	1443.4	1.0 (0.2)	1.9 (0.3)	90.0
17	DS-Tetra-DS-Tetra	1888.0	1887.8	27.1 (1.7)	25.1 (2.8)	−7.4
20	DS-Tetra-DS-Tetra anhydro	1868.1	1867.8	4.9 (0.3)	6.4 (0.5)	30.6
Other^a				16.5	13.6

"Other" muropeptides includes the summed areas of multiple small peaks that did not show significant variation between the two strains and that were not structurally characterized.

Abbreviations: DS, disaccharide (NAG-NAMol); TS, tetrasaccharide (NAG-NAM-NAG-NAMol); HS, hexasaccharide (NAG-NAM-NAG-NAM-NAG-NAMol). Tri, tripeptide (L-Ala-D-iGlu-m-Dpm); Tetra, tetrapeptide (L-Ala-D-iGlu-m-Dpm-D-Ala); Pent, pentapeptide (L-Ala-D-iGlu-m-Dpm-D-Ala-Gly). Anhydro, 1,6-anhydroNAM. The terminal NAM is in the alcohol form due to borohydride reduction except in the case of anhydro-NAM. ^cPercentages are the mean and standard error of three independent experiments.