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Changes over time in morphological parameters of hypothalamic neurons in sexually segregated cultures maintained from 1–7 days in vitro (DIV). (A) Median number of neurons at different stage of maturation: I, II, III, and IV (for references see caption in Figure 1) (B) Mean of axonal length. (C) Median number of neurons with branched neurites. Data are expressed as median or mean ± SEM. n = 5–6 independent cultures for each sex. *p < 0.050, **p < 0.010, and ***p < 0.001.
