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. Author manuscript; available in PMC: 2014 Jul 8.
Published in final edited form as: J Tissue Eng Regen Med. 2012 May 18;6(0 3):s80–s86. doi: 10.1002/term.1499

Figure 1. Light micrographs and growth curves of the stem cells on each of the 5 culture surfaces.

Figure 1

A–B) hASCs proliferated most on Lam (red line) and FN (green line), even more than on the traditional tissue culture plate (blue line). C–D) hESCs attached and proliferated well on Mat (purple line) and Lam (red line), and less well on FN (green line); hESCs did not attach to TCPS nor PLL (blue and orange lines). E) hiPS attached and grew on Mat, Lam and FN, but not on TCPS nor PLL (black arrow indicates small, singular attached stem cell; white arrow indicates previously attached cell detaching and dying); F) hiPSCs had highest cell/colony numbers on Mat (purple line), closely followed by Lam (red line); FN (green line) showed moderate iPS cell attachment initially but declined to zero by day 3; TCPS (blue line) results are hidden by PLL (orange line) results- both showed no hiPSC attachment. TCPS = tissue culture polystyrene; Lam = laminin; FN = fibronectin; Mat = Matrigel; PLL = poly-L-lysine. Scale bar = 100 μm.