Results in the numerical simulations of oxygen tension in the microfluidic device with/without a film of low diffusion coefficient of oxygen (Dfilm = 2.0×10−12 m2/s), supplying 0% oxygen gas to both gas channels (0%-0%) or supplying it to the left-hand side gas channel, while supplying 21% oxygen gas to the right-hand side (0%-21%): (a) contour map of oxygen tension at the center of the gel region on a glass cover slip, (x, y, z) = (0 m, 1.975×10−3 m, 1×10−6 m), in the microfluidic device with the film with a combination of Péclet numbers for media and gas flow in the 0%-0% case, (b) steady oxygen tension on a crosssection (z = 1.0×10−6 m) and (c) the profile across the middle of the gel region (gray shaded zone), (y, z) = (1.975×10−3 m, 1×10−6 m) at media and gas flow rates of (Pem, Peg) = (10, 100), and (d) the transient change of oxygen tension at the center of the gel region.