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Cultivation of SSCs on Matrigel in vitro. (A) and (B) Fully established SSCs on mitomycin C-treated MEFs. (C, D) SSCs cultured on Matrigel. (E) Cell number increase in Matrigel-based cultures. SSCs were seeded at a density of 5 × 105 cells/well in a 12-well plate, and cell numbers were counted for 100 days. (F) Cell proliferation rate in feeder- and Matrigel-based cultures. Scale bars, 100 μm (A, C) or 50 μm (B, D).
