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Comparison of the DNA methylation status of paternally and maternally imprinted genes between SSCs in feeder and Matrigel cultures. DNA methylation pattern of DMRs(H19, Peg1, Igf2r and Snrpn) in feeder- or Matrigel-cultured SSCs was analyzed by bisulfite sequencing. Each line represents a separate clone. Black and white circles represent methylated and unmethylated CpGs, respectively.
