Fig. 3.

LasR activity with growth restriction. (A) Two plasmids, pSC11 (lasI-lacZ fusion plasmid) and pDY101 were co-introduced into PA01 cells, and the growth of the transformed cells was finely restricted by various concentrations of arabinose. The β-galactosidase activity and growth were measured and plotted. Since lasI gene is specifically induced by LasR (Lee et al., 2006), the β-galactosidase activity reflects the LasR activity. (B, C) The growth of P. aeruginosa cells harboring only pSC11 was restricted by gentamicin (B) and tetracycline (C). The β-galactosidase activity and growth were measured and plotted in the same manner.