Fig. 5.

Effect of growth restriction on SoxR, a redox sensor protein. (A) Two plasmids, pSJ101 (PA2274-lacZ fusion plasmid) and pDY101 were co-introduced into PA01 cells and the growth of the transformed cells was restricted by various concentrations of arabinose. (B, C) The growth of P. aeruginosa cells harboring only pSJ101 was restricted by gentamicin (B) and tetracycline (C). The β-galactosidase activity and growth was measured and plotted in the same manner as in the previous figures. Since PA2274 is specifically induced by SoxR (Kobayashi and Tagawa, 2004), the β-galactosidase activity reflects the SoxR activity.